UDC

[612.63:611.664]:599.323.4

Authors

Dolgikh Olga Vasilyevna, Department of Histology, Cytology and Embryology, Northern State Medical University (Arkhangelsk, Russia)

Agafonov Yury Vitalyevich, Department of Histology, Cytology and Embryology, Northern State Medical University (Arkhangelsk, Russia)

Zashikhin Andrey Leonidovich, Department of Histology, Cytology and Embryology, Northern State Medical University (Arkhangelsk, Russia)

Abstract

Investigations of smooth muscular tissue (SMT) of rat uterus during the period of pregnancy and post partum showed that myometrium adaptation to the increasing function load was characterized by increased content of cytoplasmic protein in the smooth muscular cells. At the same time small myocytes increased in number, and proliferation potential in all parts of the uterus was enhanced. By the end of gestation, transformation of SMT in different parts of uterus was multidirectional. We found that postpartum involution of cervix myometrium is much slower than that in the horns.

Keywords

myometrium, isolated smooth myocytes, proliferation, postpartum involution, electron microscopy.

References

1. Beskov V.N. Morfologicheskie i gistokhimicheskie izmeneniya miometriya v protsesse beremennosti: avtoref. dis. … kand. med. nauk [Morphological and Histochemical Changes in the Myometrium During Pregnancy: Cand. Med. Sci. Diss. Abs.]. Karaganda, 1973. 35 p.
2. Dubinin E.V. Morfologicheskie preobrazovaniya v miometrii krys vo vremya beremennosti i v rannem poslerodovom periode: avtoref. dis. … kand. med. nauk [Morphological Changes in the Rat Myometrium During Pregnancy and Early Postpartum Period: Cand. Med. Sci. Diss. Abs.]. Novosibirsk, 2005. 30 p.
3. Kolpakova E.V. Prognozirovanie tempa poslerodovoy involyutsii matki: avtoref. dis. … kand. med. nauk [Predicting the Rate of Postpartum Involution of Uterus: Cand. Med. Sci. Diss. Abs.]. Tomsk, 2007. 26 p.
4. Shubnikova E.A., Yurina N.A., Gusev N.B., et al. Myshechnye tkani [Muscle Tissue]. Moscow, 2001. 240 p.
5. Zashikhin A.L., Agafonov Yu.V., Lisishnikov L.V. Sposob polucheniya preparatov izolirovannykh kletok [Method of Obtaining Isolated Cell Preparations]. Patent RF, no. 2104524 RU C1. Byul, 1998. no. 4.
6. Tsirkin V.I., Medvedev B.I., Grebenyuk N.G., et al. Povrezhdenie gladkomyshechnykh kletok matki zhenshchin v rodakh [Damage to the Smooth Muscle Cells of the Uterus of Women During Labour]. Akusherstvo i ginekologiya, 1986, no. 12, pp. 25–28.
7. Guide for the Care and Use of Laboratory Animals. Washington, D.C., 1996. 137 p.
8. Savitskiy G.A., Savitskiy A.G. Mioma matki (problemy patogeneza i patogeneticheskoy terapii) [Uterine Fibroid (Pathogenesis and Pathogenetic Therapy)]. 3rd ed. St. Petersburg, 2003. 236 p.
9. Shkurupiy V.A., Dubinin E.V., Dubinina H.H. Dinamika miotsitov raznykh tipov v miometrii krys v periody beremennosti i ranney poslerodovoy involyutsii [Dynamics of Different Types of Myocytes in the Rat Myometrium During Pregnancy and Early Postpartum Involution]. Byulleten’ eksperimental’noy biologii i meditsiny, 2008, suppl. no.1, pp. 101–104.
10.  Shkurupiy V.A., Dubinin E.V., Dubinina H.H. Strukturnye preobrazovaniya miotsitov v periody beremennosti i ranney poslerodovoy involyutsii matki [Structural Changes in Myocytes During Pregnancy and Early Postpartum Involution of Uterus]. Byulleten’ eksperimental’noy biologii i meditsiny, 2008, suppl. no. 1, pp. 97–100.
11. Afting E.G., Elce J.S. DNA in the Rat Uterus Myometrium During Pregnancy and Postpartum Involution. Measurement of DNA in Small Pieces of Mammalian Tissue. Analyt. Biochem., 1978, vol. 86, no. 1, pp. 90–99.
12. Shynlova O., Tsui P., Dorogin A., et al. Expression and Localization of Alpha-Smooth Muscle and Gamma- Actins in the Pregnant Rat Myometrium. Biology of Reproduction, 2005, vol. 73, no. 4, pp. 773–780.
13. Leppert P.C. Proliferation and Apoptosis of Fibroblasts and Smooth Muscle Cells in Rat Uterine Cervix Throughout Gestation and the Effect of the Antiprogesterone Onapristone. Am. J. Obstet. Gynecol., 1998, vol. 178, pp. 713–725.
14. Lye S.J. Initiation of Parturition. Anim Reprod Sci., 1996, vol. 42, pp. 495–503.
15. Shynlova O., Oldenhof A., Dorogin A., et al. Myometrial Apoptosis: Activation of the Caspase Cascade in the Pregnant Rat Myometrium at Midgestation. Biology of Reproduction, 2006, vol. 74, no. 5, pp. 839–849.
16. Report of the AVMA Panel on Euthanasia. JAVMA, 2001, vol. 218, no. 5, pp. 669–696.