UDC

612.017.2:577.352.38:612.014.46

Authors

Stryapko Nadezhda Vladimirovna, Faculty of Fundamental Medicine, Lomonosov Moscow State University (Moscow, Russia)

Sazontova Tatyana Gennadyevna, Faculty of Fundamental Medicine, Lomonosov Moscow State University (Moscow, Russia) Kostin Aleksandr Igorevich Metax Ltd. (Moscow, Russia)

Vdovina Irina Borisovna, Faculty of Fundamental Medicine, Lomonosov Moscow State University (Moscow, Russia)

ArkhipenkoYury Vladimirovich, Faculty of Fundamental Medicine, Lomonosov Moscow State University (Moscow, Russia)

Abstract

The antitoxic effects of adaptation to changing oxygen levels were investigated in the model of low dose intoxication by benzene and chromium as simulation of anthropogenic pollution. Protective effect of adaptation to hypoxia/hyperoxia is manifested in prevention of: animal weight reduction, decline in physical endurance, increase in lipid peroxidation, activation of transcription factor HIF-1α synthesis and its downstream HSPs. The results of this study demonstrate remarkable potentialities of the protective effect of adaptation to hypoxia/hyperoxia at the prenosological stage of chronic intoxication to low doses of toxicants for people working in industry or those living in the regions with anthropogenic pollution.

Keywords

adaptation, hypoxia, hyperoxia, physical endurance, redox signalling, reactive oxygen species, antioxidants, HIF-1α, HSP, benzene, potassium bichromate.

References

1. Andreeva L.I., Boykova A.A., Margulis B.A. Belki teplovogo shoka semeystva 70 kDa v otsenke sostoyaniya organizma cheloveka v norme, pri stresse i patologii [70 kDa Heat Shock Proteins in the Human Body Assessment in Health, Stress and Disease]. Estestvoznanie i gumanizm, 2009, vol. 3, no. 4, pp. 12–15.
2. Timoshinova S.V., Sharapova N.V., Mikhaylova I.V., Krasikov S.I., Boev V.M., Smolyagin V.I. Vliyanie khronicheskoy intoksikatsii khromom i benzolom na antioksidantnyy status krys [The Influence of Chronic Intoxication with Chrome and Benzol on Antioxidant Status of Rats]. Vestnik Orenburgskogo gosudarstvennogo universiteta, 2004, no. 10, pp. 132–133.
3. Ermolina E.V., Stadnikov A.A., Mikhaylova I.V., Smolyagin A.I. Issledovanie dlitel’nogo kombinirovannogo vliyaniya benzola i khroma na morfofunktsional’noe sostoyanie neyroendokrinnoy i immunnoy sistem krys Vistar [Research of the Long Combined Influence of Benzene and Chrome on Morphofunctional State of Neuroendocrine and Immune Systems of Vistar Rats]. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk, 2012, vol. 14, no. 5 (2), p. 2.
4. Platonov A.E. Statisticheskiy analiz v meditsine i biologii: zadachi, terminologiya, logika, komp’yuternye metody [Statistical Analysis in Biology and Medicine: Challenges, Terminology, Logic, Computer Methods]. Moscow, 2000. 114 p.
5. Sazontova T.G., Arkhipenko Yu.V. Rol’ svobodnoradikal’nykh protsessov i redoks-signalizatsii v adaptatsii organizma k izmeneniyu urovnya kisloroda [The Role of Free Radical Processes and Redox-Signalization in Adaptation of the Organism to Changes in Oxygen Level]. Ros. Fiziol. Zh. im. I.M. Sechenova, 2005, vol. 91, no. 6, pp. 636–655.
6. Arkhipenko Yu.V., Sazontova T.G., Glazachev O.S., Platonenko V.I. Sposob povysheniya nespetsificheskikh adaptatsionnykh vozmozhnostey cheloveka na osnove gipoksicheski-giperoksicheskikh gazovykh smesey [A Method of Increasing Non-Specific Human Adaptive Capacity Based on Hypoxic-Hyperoxic Gas Mixtures]. Patent RF, no. 2289432, 2006.
7. Utenin V.V., Stadnikov A.A., Boev V.M. Vliyanie khroma i benzola na gistostrukturu pecheni i pochek zhivotnykh v eksperimente [Effect of Chromium and Benzene on the Histological Structure of Animal Liver and Kidneys in an Experiment].Vestnik Orenburgskogo gosudarstvennogo universiteta, 2002, no. 5, pp. 10–16.
8. Boev V.M., Vereshchagin N.N., Skachkova M.A., et al. Ekologiya cheloveka na urbanizirovannykh i sel’skikh territoriyakh [Human Ecology in Urban and Rural Areas]. Orenburg, 2003, 392 p.
9. Dickinson D.A., Forman H.J. Cellular Glutathione and Thiols Metabolism. Biochem. Pharmacol., 2002, vol. 64 (5–6), pp. 1019–1026.
10. Singh M., Thomas P., Shukla D., Tulsawani R., Saxena S., Bansal A. Effect of Subchronic Hypobaric Hypoxia on Oxidative Stress in Rat Heart. Appl. Biochem. Biotechnol., 2013, vol. 169, pp. 2405–2419.
11. Fridovich I. Superoxide Dismutases: Anti- versus Pro-Oxidants? Anticancer Agents Med. Chem., 2011, vol. 11 (2), pp. 175–177.
12. Kikugawa K., Kojima T., Yamaki S., Kosugi H. Interpretation of the Thiobarbituric Acid Reactivity of Rat Liver and Brain Homogenates in the Presence of Ferric Ion and Ethylenediaminetetraacetic Acid. Analyt. Biochem., 1992, vol. 202, pp. 249–255.
13. Myers C.R. The Effects of Chromium (VI) on the Thioredoxin System: Implications for Redox Regulation. Free Radic. Biol. Med., 2012, vol. 52, no. 10, pp. 2091–2107.
14. Sazontova T.G., Arkhipenko Y.V. Intermittent Hypoxia in Resistance of Cardiac Membrane Structures: Role of Reactive Oxygen Species and Redox Signaling. Intermittent Hypoxia: From Molecular Mechanisms to Clinical Applications. Ed. by Xi L., Serebrovskaya T.V. New York, 2009, pp. 147–187.
15. Suematsu M., Ishimura Y. The Heme Oxygenase-Carbon Monoxide System: A Regulator of Hepatobiliary Function. Hepatology, 2000, vol. 31, pp. 3–6.
16. Barreto G., Madureira D., Capani F., Aon-Bertolino L., Saraceno E. The Role of Catechols and Free Radicals in Benzene Toxicity: An Oxidative DNA Damage Pathway. Environmental and Molecular Mutagenesis, 2009, vol. 50, pp. 771–780.
17. Zhukova A.G., Sazontova T.G. Heme Oxygenase: Function, Regulation, Biological Role. Hypoxia Med. J., 2004, no. 3, pp. 30–43.