UDC

612.014:612.017.1

Authors

Dobrodeeva Liliya Konstantinovna
The Institute of Environmental Physiology, Ural Branch of the Russian Academy of Sciences (Arkhangelsk, Russia)
Patrakeeva Veronika Pavlovna
The Institute of Environmental Physiology, Ural Branch of the Russian Academy of Sciences (Arkhangelsk, Russia)
Balashova Svetlana Nikolaevna
The Institute of Environmental Physiology, Ural Branch of the Russian Academy of Sciences (Arkhangelsk, Russia)

Abstract

The paper presents the results of immunoassays of healthy working-age people aimed to determine the relationship between lymphoproliferation and key parameters of immune status. We compared and analyzed immune responsiveness in patients with malignant tumours of epithelial origin, atopic dermatitis and type II diabetes. In all of the cases, lymphoproliferation is associated with increasing concentrations of natural killers, T- lymphocytes with transferrin receptor and total neutrophil granulocytes. Moreover, activation of myeloid cells is accompanied by an increase in total circulating lymphocytes. In patients with cancer accompanied by lymphopenia, proliferation was more pronounced; this applies not only to lymphocytes and neutrophils but also to monocytes. Patients with type II diabetes mellitus had, besides high levels of CD10+ and CD71+, a rapid increase in natural killers. In atopic dermatitis, lymphoproliferation of B-lymphocytes (CD10+) is associated with the content of IgE and rate of monocyte proliferation.

Keywords

proliferation, lymphocytes, neutrophils, monocytes, immune response

References

1. Sibiryak S.V., Razumnaya F.G., Salimgareeva M.Kh., et al. Vliyanie afobazola na proliferativnuyu aktivnost’ limfotsitov perifericheskoy krovi v sisteme in vitro [Influence of Afobazole on the Peripheral Blood Lymphocytes Proliferative Activity in vitro]. Rossiyskiy immunologicheskiy zhurnal, 2009, vol. 3 (12), no. 1, pp. 50−57. 
2. Nikolaev S.B., Bystrova N.A., Lazarenko V.A., Konoplya A.I. Immunometabolicheskie narusheniya v usloviyakh gipoksii i ikh farmakologicheskaya korrektsiya [Immune and Metabolic Disorders at Hypoxia and Their Pharmacological Treatment]. Kursk, 2010. 224 p. 
3. Harris N.L., Jaffe E.S., Stein H., et al. A Revised European-American Classification of Lymphoid Neoplasms: A Proposal from the International Lymphoma Study Group. Blood, 1994, vol. 84, p. 1361. 
4. Greaves M., Brown G., Rapson N., Lister T. Antisera to Acute Lymphoblastic Leukemia Cells. Clin. Immunol. Immunopathol., 1975, vol. 4, no. 1, p. 67. 
5. Becker E.L., Henson P.M. In vitro Studies of Immunologically Induced Secretion of Mediators from Cells and Related Phenomena. Adv. Immunol., 1973, vol. 17, pp. 93−193. 
6. Betts M.R., Brenchley J.M., Price D.A., et al. Sensitive and Variable Identification of Antigen-Specific CD8+T cells by a Flow Cytometric Assay for Degranulation. J. Immunol. Methods, 2003, vol. 281, no. 1–2, pp. 65−78. 
7. Rossi M.I., Yokota T., Medina K.L., et al. B lymphopoiesis Is Active Throughout Human Life, but There Are Developmental Age-Related Changes. Blood, 2003, vol. 101 (2), pp. 576−584. 
8. Casazza J.P., Betts M.R., Price D.A., et al. Acquisition of Direct Antiviral Effector Functions by CMV-Specific CD4+ T Lymphocytes with Cellular Maturation. J. Exp. Med., 2006, vol. 203 (13), pp. 2865−2877. 
9. Metzgar R.S., Borowitz M.J., Jones N.H., Dowell B.L. Distribution of Common Acute Lymphoblastic Leukemia Antigen in Nonhematopoietic Tissues. J. Exp. Med., 1981, vol. 154 (4), pp. 1249−1254. 
10. Ritz J., Nadler L., Bhan A., et al. Expression of a Common Acute Lymphoblastic Leukemia Antigen (CALLA) by Lymphomas of B-cell and T-cell Lineage. Blood, 1981, vol. 58 (3), p. 648. 
11. Gale D.P., Maxwell P.H. The Role of HIF in Immunity. Int. J. Biochem. Cell Biol., 2010, vol. 42 (4), pp. 486−494. 
12. Gerriets V.A., Rathmell J.C. Metabolic Pathways in T Cell Fate and Function. Trends Immunol., 2012, vol. 33 (4), pp. 168−173. 
13. Goldstein J.L. Genetic Aspects of Hyperlipidemia in Coronary Heart Disease. Hosp. Practice, 1973, pp. 53−65. 
14. Henson P.M. Pathologic Mechanism in Neutrophil-Mediated Injury. Am. J. Pathol., 1972, vol. 68 (3), pp. 593−606. 
15. Lotner G.Z., Lynch J.M., Betz S.J., Henson P.M. Human Neutrophil-Derived Platelet Activating Factor. J. Immunol., 1980, vol. 124 (2), pp. 646−684. 
16. Los M., Schenk H., Hexel K., et al. IL-2 Gene Expression and NF-kappa B Activation Through CD28 Requires Reactive Oxygen Production by 5-lipoxygenase. EMBO J., 1995, vol. 14 (15), pp. 3731−3740. 
17. Kominsky D.J., Campbell E.L., Colgan S.P. Metabolic Shifts in Immunity and Inflammation. J. Immunol., 2010, vol. 184 (8), pp. 4062−4068. 
18. Lavrentieva A., Majore I., Kasper K., Hass R. Effects of Hypoxic Culture Conditions on Umbilical Cord-Derived Human Mesenchymal Stem Cells. Cell Communication and Signaling, 2010, vol. 8 (1), pp. 18−25. 
19. Meyron-Holtz E.G., Ghosh M.C., Rouault T.A. Mammalian Tissue Oxygen Levels Modulate Iron-Regulatory Protein Activities in vivo. Science, 2004, vol. 306 (5704), pp. 2087−2090. 
20. Nekanti U., Dastidar S., Venugopal P., et al. Increased Proliferation and Analysis of Differential Gene Expression in Human Wharton’s Jelly-Derived Mesenchymal Stromal Cells Under Hypoxia. Cell, 2010, vol. 6 (5), pp. 499−521. 
21. Yun S., Lee S.H., Yoon S.-R., et al. Oxygen Tension Regulates NK Cells Differentiation from Hematopoietic Stem Cells in vitro. Immunol. Lett., 2011, vol. 137 (1–2), pp. 70−77. 
22. Rolfs A., Kvietikova I., Gassmann M., Wenger R.H. Oxygen-Regulated Transferrin Expression Is Mediated by Hypoxia-Inducible Factor-1. J. Biol. Chem., 1997, vol. 272 (32), pp. 20055−20062. 
23. Post J., Hoffman J. Сell Renewal Patterns. New Engl. J. Med., 1968, vol. 279 (5), pp. 248−258. 
24. Greaves M., Hariri G., Newman R., et al. Selective Expression of the Common Acute Lymphoblastic Leukemia (gp100) Antigen on Immature Lymphoid Cells and Their Malignant Counterparts. Blood, 1983, vol. 61, p. 628. 
25. Shipp M., Look A. Hematopoietic Differentiation Antigens That Are Membrane-Associated Enzymes: Cutting Is the Key! Blood, 1993, vol. 82 (4), p. 1052. 
26. Thiery J., Keefe D., Boulant S., et al. Perforin Pores in the Endosomal Membrane Trigger the Release of Endocytosed Granzyme B into the Cytosol of Target Cells. Nat. Immunol., 2011, vol. 12 (8), pp. 770−777. 
27. Wright D.G., Gallin J. A Functional Differentiation of Human Neutrophil Granules: Generation of C5a by a Specific (Secondary) Granule Product and Inactivation of C5a by Azurophil (Primary) Granule Products. J. Immunol., 1977, vol. 119 (3), pp. 1068−1076.