UDC

612.1:576.52

Authors

Elena A. Shamray*, Marina Yu. Skorkina*, Evgeniya A. Sladkova*
*Belgorod National Research University (Belgorod, Russian Federation)
Corresponding author: Elena Shamray, address: ul. Studencheskaya 14, Belgorod, 308007, Russian Federation;
e-mail: elenashamray@yandex.ru

Abstract

Chronic myeloproliferative diseases occupy an important place among pathophysiological conditions of the circulatory system. Their progress from the early stages is accompanied by changes in lymphocyte structural and functional properties. This paper aimed to study the elastic and adhesive properties of lymphocytes in healthy individuals and patients with chronic myelogenous leukaemia at the stages of treatment and relapse. New data on the functional properties of normal and abnormal lymphocyte cells were obtained. The results of the study showed that in patients at the treatment stage the stiffness of the cell surface increased by a factor of 2.8, while adhesion in the “lymphocyte–leukocyte” and “lymphocyte–erythrocyte” systems decreased by 33.7 % and 38.5 % (p < 0.05), respectively, compared with healthy subjects. In patients with chronic myelogenous leukaemia at the relapse stage, the stiffness of the plasma membrane doubled, while the adhesive strength in the “lymphocyte-leukocyte” system increased by 5.2 % (p < 0.05), and in the “lymphocyte-erythrocyte” system it decreased by 10.3 % (p < 0.05), compared with the control. Due to increased rigidity of their surface during the development of chronic myeloproliferative diseases, the cells are unable to change their form when passing through small capillaries, which results in lower motor activity of lymphocytes and can damage the microcirculation system as they move through the vessels. Disturbed adhesive interactions change the cell invasive potential. Thus, the obtained data are of practical importance for the diagnosis of cytological disorders and prognosis of the course of the disease.

Keywords

lymphocytes, chronic myelogenous leukaemia, lymphocyte elastic properties, lymphocyte adhesion, atomic force microscopy, biosensor chip

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