UDC

616.314.17-[002.2-031.81-085.847.8-085.849.19]-036.8(045)

Authors

Vladimir V. Maslyakov*, Elena A. Pronina*, Yuliya V. Abakumova*, Anatoliy V. Il’yukhin*
*Reaviz Saratov Medical University (Saratov, Russian Federation)

Abstract

This paper aimed to study the normalizing effect of extremely high frequencies (EHF) on the microbiota in various forms of generalized chronic periodontitis. The research involved 160 patients with generalized chronic periodontitis (mean age 30 years), who underwent complete medical examination, including the microbiological analysis of the periodontal pocket contents. The research established that a significant role in the pathogenesis of mild periodontitis is played by the stabilizing microbiota and the aggressive part of the stabilizing microbiota, while in case of moderate periodontitis, by the aggressive part of the stabilizing microbiota. To improve the results of treatment and correction of oral microbiota, we suggested using 10 sessions of EHF-therapy directed at the biologically active points on the face (according to the method developed earlier). These frequencies stabilized the microbiota in patients with moderate periodontitis on the 7th day of the therapy: the stabilizing microbiota accounted for 55 %, the aggressive part of the stabilizing microbiota, for 40 %, and type 1 pathogens (Prevotella intermedia, Bacteroides forsythus, Treponema denticola, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis), for 5 % of the total microbiota. In the control group, who did not receive EHF-therapy, the stabilizing microbiota accounted for 35 %, the aggressive part of the stabilizing microbiota, for 45 %, type 1 pathogens, for 12 %, and type 2 pathogens (Streptococcus intermedius and Actinomyces spp.), for 8 % of the total microbiota. The total microbial count in the main group (after EHF-therapy) decreased from 13.4 ± 1.6 to 9.4 ± 1.3, while in the control group, only to 11.1 ± 1.6. Patients with mild periodontitis showed improvements as early as on the 5th day of therapy: the amount of stabilizing microbiota reached 75 %, the aggressive part of the stabilizing microbiota amounted to 21 %, and type 1 pathogens, to 4 % (in the control group, the number of the microorganisms was 50, 36 and 14 %, respectively); the total microbial count remained unchanged: 9.23 ± 1.2. Thus, the use of EHF-therapy in combination therapy of generalized chronic periodontitis facilitates the restoration of oral microbiota.

Keywords

generalized chronic periodontitis, extremely high frequencies, periodontal pocket microbiota, correction of oral microbiota

References

1. Anurova A.E., Velichko E.V., Kosyreva T.F., Sturov N.V. Vliyanie mikroflory polosti rta materey na formirovanie mikrobiotsenoza polosti rta u detey s vrozhdennymi rasshchelinami verkhney guby i neba [Influences of Maternal Oral Microflora on Specific Characteristics of Oral Microbiocenosis in Children with Congenital Cleft Lip and Palate]. Trudnyy patsient, 2017, vol. 15, no. 1-2, pp. 59–63. 
2. Vostrikova S.A., Lepilin A.V., Karabushina Ya.G., Makletsova E.K. Primenenie ul’trazvukovoy sistemy “VECTOR” v lechenii patsientov s khronicheskim generalizovannym parodontitom [The Use of Ultrasonic System “Vector ” in Treatment of Chronic Generalized Periodontitis]. Saratovskiy nauchno-meditsinskiy zhurnal, 2008, no. 2, pp. 132–136. 
3. Molokov V.D., Vasil’eva L.S., Tirskaya O.I., Kal’k E.A. Effektivnost’ vliyaniya dializa linkomitsina na mikrofloru parodontal’nogo karmana u bol’nykh khronicheskim generalizovannym parodontitom legkoy i sredney stepeni tyazhesti [Effectiveness of Lincomycin Dialysis Influence on the Periodontal Pocket Microflora in Patients with a Mild and Severe Forms of Chronic Generalized Periodontitis]. Sibirskiy meditsinskiy zhurnal, 2009, no. 8, pp. 19–21. 
4. Morgunova V.M. Mikrobiologicheskaya kharakteristika soderzhimogo parodontal’nykh karmanov bol’nykh parodontitom [Microbiological Characteristics of the Contents of Periodontal Pockets of Patients with Periodontitis]. Saratovskiy nauchno-meditsinskiy zhurnal, 2011, vol. 7, no. 1 (suppl.), pp. 312–314. 
5. Kassie F., Darroudi F., Kundi M., Schulte-Hermann R., Knasmüller S. Khat (Catha edulis) Consumption Causes Genotoxic Effects in Humans. Int. J. Cancer, 2001, vol. 92, no. 3, pp. 329–332. 
6. Siegrist B.E., Gusberti F.A., Brecx M.C., Weber H.P., Lang N.P. Efficacy of Supervised Rinsing with Chlorhexidine Digluconate in Comparison to Phenolic and Plant Alkaloid Compounds. J. Periodont. Res., 1986, vol. 21, no. s16, pp. 60–73. 
7. Tamarova E.R., Mavzyutov A.R. Osobennosti mikroflory polosti rta u bol’nykh parodontitom [Features of Microflora of Oral Cavity in Patients with Periodontitis]. Byulleten’ Orenburgskogo nauchnogo tsentra UrO RAN (elektronnyy zhurnal), 2013, no. 3, pp. 1–5. Available at: http://elmag.uran.ru:9673/magazine/Numbers/2013-3/Articles/ Tamarova-Mavzyutov(2013-3).pdf (accessed 23 January 2018). 
8. Tamarova E.R., Masagutova N.R. Molekulyarno-geneticheskaya kharakteristika mikroflory polosti rta pri parodontite [Molecular-Genetic Characteristics of Oral Cavity Microflora in Periodontitis]. Vestnik Chelyabinskogo gosudarstvennogo universiteta, 2013, no. 7: Biologiya, iss. 2, pp. 70–71. 
9. Tsepov L.M., Morozov V.G., Nikolaev A.I. Nereshennye voprosy etiologii i patogeneza vospalitel’nykh zabolevaniy parodonta [Unsolved Questions of Aetiology and Pathogenesis of Inflammatory Periodontal Diseases]. Parodontologiya, 2001, no. 1-2, pp. 28–31. 
10. Järvensivu A., Hietanen J., Rautemaa R., Sorsa T., Richardson M. Candida Yeasts in Chronic Periodontitis Tissues and Subgingival Microbial Biofilms in vivo. Oral Dis., 2004, vol. 10, no. 2, pp. 106–112. 
11. Shumskiy A.V. Immunopatogeneticheskiy podkhod v lechenii vospalitel’nykh zabolevaniy polosti rta [The Immunopathogenetic Approach in Treatment of Inflammatory Diseases of an Oral Cavity]. Paradontologiya, 2005, no. 4, pp. 12–15. 
12. Löe H., Schiøtt C.R. The Effect of Mouthrinses and Topical Application of Chlorhexidine on the Development of Dental Plaque and Gingivitis in Man. J. Periodontal Res., 1970, vol. 5, no. 2, pp. 79–83. 
13. Orekhova L.Yu. Zabolevaniya parodonta [Periodontal Diseases]. Moscow, 2004. 432 p. 
14. Ivanov V.S. Zabolevaniya parodonta [Periodоntal Diseases]. Mosсow, 2001. 300 p. 
15. Shirokov V.Yu., Danilov A.S., Zhdanova O.Yu., Titorenko V.A. Polovoy dimorfizm izmeneniya funktsiy endoteliya sosudistoy stenki pri khronicheskom generalizovannom parodontite v dinamike kompleksnogo lecheniya s ispol’zovaniem KVCh-terapii [Sexual Dimorphism in Changes of Vascular Endothelial Function at Periodontitis in the Dynamics of the Treatment with the Use of EHF-Therapy]. Problemy stomatologii, 2014, no. 2, pp. 31–33.