UDC

615.241:591.112.1

Authors

Irina S. Matyul’ko*, Adil’ A. Bayzhumanov**, Elizaveta E. Khirazova**, Mariya V. Maslova**
*Р.K. Anokhin Research Institute of Normal Physiology (Moscow, Russian Federation)
**Lomonosov Moscow State University (Moscow, Russian Federation)
Corresponding author: Irina Matyul’ko, address: ul. Baltiyskaya 8, Moscow, 125315, Russian Federation; e-mail: irinamatulko@gmail.com

Abstract

Nowadays, many people around the world have limited access to pure drinking water. At the same time, a constant hydration level is essential for normal functioning and maintenance of homeostasis in the body systems. In this study we investigated the effects of two regimes of water deprivation on the activity of the blood antioxidant defence system, processes of free-radical oxidation, and behaviour in rats. Male Wistar rats (n = 50) were deprived of water for 96 hours with ad libitum access to food. After the deprivation period, we assessed their behaviour in the open field test and measured the activity parameters of the blood antioxidant defence system. Behaviour was evaluated by the parameters of orientation and exploratory activity, level of anxiety, and locomotor activity. The activity of the antioxidant defence system was assessed by the level of total antioxidant activity in the blood plasma, catalase activity in blood haemolysate, and activity of cytoplasmic and extracellular forms of Cu,Zn-superoxide dismutase. In addition, we measured the level of oxidative stress markers and the content of nonenzymic antioxidants in the blood plasma contributing to the maintenance of cellular redox status. The levels of blood haemoglobin and total protein in the plasma were evaluated as indicators of metabolism. The result of the study showed that 96-hour water deprivation leads to changes in anxiety level reflected in a decrease in the number of grooming acts and total grooming time. Changes in the parameters of the blood antioxidant defence system were observed, including changes in haemoglobin level, plasma ceruloplasmin concentration, total antioxidant activity in the plasma, and blood catalase activity. Therefore, the research demonstrated that long-term water deprivation activates the blood antioxidant defence system in response to oxidative stress and leads to inflammation. What is more, dehydration stress induces anxiety-like behaviour in animals.

Keywords

water deprivation, dehydration stress, blood antioxidant defence system, behaviour

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