UDC

616-036.882-08:615.2

Authors

Petr P. Zolin*, Vladimir D. Konvay*/**
*Omsk State Medical University (Omsk, Russian Federation)
**Omsk State Agrarian University named after P.A. Stolypin (Omsk, Russian Federation)

Abstract

Resuscitation science is currently searching for new ways to treat post-resuscitation energy deficits and restore human brain functions. This paper aimed to test the ability of exogenous D-(–)-ribose to influence the metabolism of free nucleotides in rat brain and the neurological status of rats in the early post-resuscitation period. Experiments were carried out on male rats exposed to 6.5 min asphyxia followed by resuscitation. Rats were narcotized with ether 30 min after resuscitation, and their brains were placed in liquid nitrogen ex vivo. The control animals were subjected to the same procedures except for asphyxia and resuscitation. We found enhanced catabolism of free nucleotides in the brain of resuscitated rats, as evidenced by their reduced content; namely, the concentration of nucleoside di- and triphosphates (p < 0.05) and nucleoside monophosphates decreased (p < 0.005) as compared with the control group. Intravenous injection of D-(–)-ribose (50 mg/kg body weight) immediately after resuscitation had a beneficial effect on nucleoside di- and triphosphates concentration in the brain. The authors suppose that it is associated with phosphorylation of ribose to ribose-5-phosphate, which is essential for phosphoribosyl diphosphate formation. The latter provides the inclusion of nucleobases in salvage pathway and nucleotide synthesis de novo. In addition, the authors believe that the positive effect of ribose is due to the use of ribose-5-phosphate as an energy substrate through the formation of glyceraldehyde-3-phosphate. As a result, injection of ribose in rats immediately after resuscitation leads to a 20 % decrease in the sum scores of neurological deficit (p < 0.05) and a twofold decrease in the share of maximal scores (p < 0.05).

Keywords

ribose, nucleoside triphosphates, nucleoside diphosphates, nucleoside monophosphates, resuscitation, brain

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