UDC

616.155.291

DOI

10.17238/issn2542-1298.2019.7.3.280

Authors

Aleksandr V. Solomennikov*, Aleksandr I. Tyukavin**, Nikolay A. Arseniev**
*Almazov National Medical Research Centre (St. Petersburg, Russian Federation)
**Saint Petersburg State Chemical and Pharmaceutical University (St. Petersburg, Russian Federation)
Corresponding author: Aleksandr Solomennikov, address: ul. Akkuratova 2, St. Petersburg, 197341, Russian Federation; e-mail: Solomen33@mail.ru

Abstract

This paper studied the dynamics of structural changes in leukogram ratio panels of 18 patients with high platelet counts using the author’s method of data processing and analysis within the framework of the data mining strategy. The authors make a general conclusion that distinctive signs of deformation of the leukocyte ratio panel against the background of platelet growth and degranulation may indicate both pro-inflammatory and anti-inflammatory activity of granule secretion. In the first case, the increase in platelet number and degranulation were accompanied by greater activity of C-reactive protein, growing number of neutrophils against the background of leukocyte-associated increase in vascular permeability and greater cytokine activity of neutrophils; in the second case, they were accompanied by a decline in the number of neutrophils, lower activity of C-reactive protein against the background of stabilization of vascular permeability and a decrease in cytokine activity, which, presumably, contributes to the “erasure” and remodelling of adhesive molecules earlier expressed on the endothelium. These results are consistent with the latest literature data suggesting that degranulation of the secretion of dense and α-granules corresponds to the activation of pro-inflammatory response in leukocytes, while the secretion of ɣ-granules (lysosomes) has no such properties. In the surveyed patients, “reparative” platelet-associated changes in the leukogram structure were manifested as either leading or highly important, while pro-inflammatory changes produced no statistically significant effect on the leukogram structure. The latter finding indicates an important independent role of ɣ-granule degranulation in local reparative processes, while at the peak of inflammation the biological effects of degranulation of dense and α-granules are realized in combination with other pro-inflammatory factors (e.g. high activity of individual leukocyte subpopulations and of the complement system, etc.).

Keywords

platelet degranulation, leukogram structure, inflammatory process

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