UDC

577.175.52:[612.82+612.3]

DOI

10.17238/issn2542-1298.2019.7.4.381

Authors

Emiliya T. Gadzhieva*  ORCID: 0000-0002-4780-4466
Mamed A. Karaev* ORCID: 0000-0001-6862-1653
*Azerbaijan State Pedagogical University (Baku, Republic of Azerbaijan)
Corresponding author: Emiliya Gadzhieva, address: 68 Uzeyir Hajibeyli St., AZ1000, Baku, Republic of Azerbaijan; e-mail: nazaket-alieva@mail.ru

Abstract

This paper aimed to study norepinephrine levels in the mitochondria of the brain structures of 3-month-old rabbits with different periods of water deprivation and against the background of restored drinking regime. The experimental group was divided into 4 subgroups: rabbits exposed to 1, 3 and 5 days of water deprivation and those exposed to 5 days of water deprivation followed by 7 days of restored drinking regime. To determine norepinephrine levels we used the universal fluorimetric method. The reference mitochondrial fraction of the brain was isolated according to the method of Somogyi and Fonjo. The research found that forced water deprivation causes an increase in norepinephrine levels in the mitochondrial fractions of the brain in 3-month-old rabbits. This increase depends on the duration of water deprivation and on the brain structure. After one day of water deprivation, norepinephrine levels in different brain structures increase by 17–29 %, compared with the control animals. On the 3rd and 5th days of water deprivation, norepinephrine levels increase by 25–36 % and 33–47 %, respectively, compared with the control. The effect of water deprivation on the hypothalamic noradrenergic systems is generally more pronounced, compared with other brain structures under study (orbitofrontal, sensorimotor, limbic, visual cortex and brainstem). Against the background of 7 days of restored drinking regime, norepinephrine levels tend to decrease in all the above-mentioned brain structures. The research results indicate that, depending on the duration of water deprivation, norepinephrine levels in rabbit brain structures undergo significant changes.

Keywords

dehydration stress, norepinephrine, water deprivation, drinking regime, rabbits

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