UDC

612.111:612.017.1:612.014.43

DOI

10.17238/issn2542-1298.2019.7.4.427

Authors

Anna V. Samodova* ORCID: 0000-0001-9835-8083
Liliya K. Dobrodeeva* ORCID: 0000-0001-5080-6502
*N. Laverov Federal Center for Integrated Arctic Research, Russian Academy of Sciences (Arkhangelsk, Russian Federation)
Corresponding author: Anna Samodova, address: prosp. Lomonosova 249, Arkhangelsk, 163061, Russian Federation; e-mail: annapoletaeva2008@yandex.ru

Abstract

This research involved 108 healthy subjects living in the city of Arkhangelsk and aged from 21 to 50 years (69 women and 39 men) before and after staying in a climate chamber for 5 minutes at –25 ºC. Using the haematological analyser XS-500i Sysmex (Japan) we studied red blood cell and platelet parameters as well as haematocrit levels in the peripheral venous blood. Erythrocyte aggregation was determined by microscopy of diluted blood according to the method of V.A. Levtov, S.A. Regirer and I.Kh. Shadrina (1982). We studied haemograms, neutrograms, monocytograms and lymphocytograms in blood film stains according to the Romanovsky-Giemsa method, as well as the phagocytic activity of neutrophils, and lymphocyte phenotypes (CD3+, CD4+, CD8+, CD10+, CD19+, CD23+, CD71+, CD95+) using the method of indirect immunoperoxidase reaction. The levels of IL-1β, TNF-α and IL-6 cytokines, irisin, and endothelin-1 were determined by the enzyme-linked immunosorbent assay. The majority of the examined individuals (70.37 %) showed no differences in haematocrit levels, erythrocyte content or haemoglobin before and after short-term cooling. In 23.15 % of the subjects, after the climate chamber the haematocrit level increased concurrently with the growing erythrocyte and haemoglobin levels; in 6.48 % of cases, the proportion of blood volume occupied by erythrocytes decreased. The rise in haematocrit levels is associated with increasing platelet concentration. In 20 % of cases, after the climate chamber the examined individuals showed an increase in red blood cell aggregation and in the average size of erythrocyte aggregates with a concurrent decrease in the share of non-aggregated erythrocytes. It was established that an increase in blood viscosity at cooling is combined with a noticeable decrease in the content of all types of lymphocytes and their phenotypes due to redistribution of myeloid cells from the circulating pool to the marginal pool with the participation of endothelin-1 vasoconstrictor.

Keywords

haematocrit, erythrocyte aggregation, platelets, neutrophils, lymphocytes, endothelin-1, irisin, cold exposure

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