UDC

612.017:613.166.9:612.084:612.42

DOI

10.17238/issn2542-1298.2019.7.4.436

Authors

Ol’ga A. Stavinskaya* ORCID: 0000-0002-0022-5387
Veronika P. Patrakeeva* ORCID: 0000-0001-6219-5964 
*N. Laverov Federal Center for Integrated Arctic Research, Russian Academy of Sciences (Arkhangelsk, Russian Federation)
Corresponding author: Ol’ga Stavinskaya, address: prosp. Lomonosova 249, Arkhangelsk, 163000, Russian Federation; e-mail: ifpa-olga@mail.ru

Abstract

This research aimed to identify lymphocyte phenotypes that are particularly susceptible to programmed death in apparently healthy people. We examined 138 subjects aged from 20 to 60 years living and working in the Arkhangelsk Region (Russia). Peripheral blood leukograms were determined using the Sysmex XS-500i haematology analyser (Japan). Lymphocyte apoptosis was assessed on the Epics XL Flow Cytometer (Beckman Coulter, USA). The concentrations of cytokines and mediators of apoptosis in the blood were estimated using the solid-phase enzyme immunoassay. Lymphocytograms were studied by means of microscopy (Vision MT5300L, Japan) in blood film stains according to the Romanovsky– Giemsa method. The lymphocyte phenotypes content was estimated by double peroxidase staining using monoclonal antibodies. The limits of normal distribution of quantitative indices were determined by means of the Shapiro–Wilk test. Statistical significance of the differences between the groups was estimated by means of the Student’s t-test and the Wilcoxon signed-rank test. The results were analysed by comparing the levels of responses depending on the count of apoptotic lymphocytes AnV+/PI- in the blood: group 1 had a rather low count, <0.05·109 kl/l (n = 46); group 2 had a count ranging between 0.05 and 0.1·109 kl/l (n = 35); group 3 had a rather high count, >0.1·109 kl/l (n = 57). It was established that in subjects with the maximum count of apoptotic cells, the total lymphocyte count is higher due to CD3+, CD8+, CD10+, CD16+, CD23+, CD25+, CD71+ and HLA-DR against the background of decreased concentration of T helper cells (CD4+) and necrotized lymphocytes. Thus, energy-dependent activation of lymphocyte apoptosis is associated with lymphocyte activation, proliferation and differentiation, while programmed death primarily affects T helper cells.

Keywords

lymphocyte apoptosis, lymphocyte phenotypes, cytokines, apparently healthy people

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