UDC

574.24:576.7

DOI

10.37482/2687-1491-Z030

Authors

Parizat M. Nurmagomedova* ORCID: 0000-0002-4207-8880
Mar’yam G. Omarieva* ORCID: 0000-0003-1649-2508
Bashir G. Magomedgadzhiev** ORCID: 0000-0002-3822-6895
*Dagestan State University (Makhachkala, Republic of Dagestan, Russian Federation)
**Dagestan State Medical University (Makhachkala, Republic of Dagestan, Russian Federation)
Corresponding author: Parizat Nurmagomedova, address: ul. M. Gadzhieva 43-a, Makhachkala, 367000, Respublika Dagestan, Russian Federation; e-mail: Parizat1949@bk.ru

Abstract

Histological preparations of ground squirrel liver, heart and brain tissues were studied in the middle of the hibernation bout (body temperature of 1–4 °C), at self-heating (body temperature of 6–29 °C) and upon complete awakening (body temperature of 37–38 °C). In the state of deep hibernation, no signs of apoptosis were identified in ground squirrel tissues. We observed microcirculatory dysfunction manifested in abnormal blood rheology: acute hyperaemia of brain and myocardial capillaries, central veins and sinusoids of the liver, as well as mild tissue oedema and aggregations of red blood cells (rouleaux) in microvasculature. At self-heating, signs of the initial stage of apoptosis were detected: mild granularity in cardiomyocyte cytoplasm as a sign of protein dystrophy, and mild cytoplasmic vacuolization in hepatocytes. Morphological signs of these processes were revealed upon the animal’s complete awakening. Shrinkage of individual hepatocytes was observed; cells with pyknotic nuclei appeared, which can be explained by the condensation of chromatin and intracellular organelles; cell fragments with karyorrhectic phenomena resembling apoptotic bodies were identified. Thus, no signs of apoptosis were found in ground squirrel tissues during deep hibernation. At self-heating, signs of the initial stage of apoptosis were detected, and only in the state of complete awakening were single hepatocytes and neurons similar to apoptotic bodies identified. In addition, the absence of an inflammatory reaction in the tissues under study allows us to suggest that we are observing the stages of apoptosis. Noteworthy, ground squirrel awakening was accompanied by a significant increase in the activity of lysosomal membrane markers cathepsin D and acid phosphatase.

Keywords

hibernation, ground squirrels, apoptosis, lysosomes, cathepsin D.

References

1. Nurmagomedova P.M., Pashaeva M.E., Khizrieva S.S., Dzhanbolatova D.N. Sostoyanie lizosomal’nykh membran i aktivnost’ fermentov lizosom pri zimney spyachke i probuzhdenii [The State of Lysosomal Membranes and the Activity of Lysosomal Enzymes During Hibernation and Awakening]. Modernizatsiya nauki i obrazovaniya [Modernization of Science and Education]. Makhachkala, 2015, pp. 120–1 25.
2. Gusakova E.A., Gorodetskaya I.V. Stress i proteoliticheskie fermenty lizosom [Stress and Proteolytic Lysosomal Enzymes]. Vestnik Vitebskogo gosudarstvennogo meditsinskogo universiteta, 2012, vol. 11, no. 4, pp. 15–25.
3. Pupyshev A.B. Permeabilizatsiya lizosomnykh membran kak apoptogennyy faktor [Lysosomal Membrane Permeabilization as Apoptogenic Factor]. Tsitologiya, 2011, vol. 53, no. 4, pp. 313–324.
4. Repnik U., Hafner Česen M., Turk B. Lysosomal Membrane Permeabilization in Cell Death: Concepts and Challenges. Mitochondrion, 2014, vol. 19, pt. A, pp. 49–57.
5. Roberg K., Kågedal K., Öllinger K. Microinjection of Cathepsin D Induces Caspase-Dependent Apoptosis in Fibroblasts. Am. J. Pathol., 2002, vol. 161, no. 1, pp. 89–96.
6. Yang M., Zhang M., Tahara Y., Chechetka S., Miyako E., Iijima S., Yudasaka M. Lysosomal Membrane Permeabilization: Carbon Nanohorn-Induced Reactive Oxygen Species Generation and Toxicity by This Neglected Mechanism. Toxicol. Appl. Pharmacol., 2014, vol. 280, no. 1, pp. 1 17–126.
7. Serrano-Puebla A., Boya P. Lysosomal Membrane Permeabilization in Cell Death: New Evidence and Implications for Health and Disease. Ann. N. Y. Acad. Sci., 2016, vol. 1371, no. 1, pp. 30–44.
8. Repnik U., Stoka V., Turk V., Turk B. Lysosomes and Lysosomal Cathepsins in Cell Death. Biochim. Biophys. Acta, 2012, vol. 1824, no. 1, pp. 22–33.
9. Yarovaya G.A., Neshkova E.A., Martynova E.A., Blokhina T.B. Rol’ proteoliticheskikh fermentov v kontrole razlichnykh stadiy apoptoza [The Role of Proteolytic Enzymes in the Control of Different Stages of Apoptosis]. Laboratornaya meditsina, 2011, no. 11, pp. 39–52.
10. Rossi A., Lord J.M. Adiponectin Inhibits Neutrophil Apoptosis via Activation of AMP Kinase, PKB and ERK 1/2 MAP Kinase. Apoptosis, 2013, vol. 18, no. 12, pp. 1469–1480.
11. Bocharova L.S., Gordon R.Ya., Rogachevskiy V.V., Ignat’ev D.A., Khutsyan S.S. Tsiklicheskie izmeneniya struktury endoplazmaticheskogo retikuluma i apparata Gol’dzhi neyronov gippokampa suslikov vo vremya zimney spyachki [Cyclic Structural Changes in Endoplasmic Reticulum and Golgi Complex in the Hippocampal Neurons of Ground Squirrels During Hibernation]. Tsitologiya, 2011, vol. 53, no. 3, pp. 259–269.