UDC

612.115.12:[57.042.05.577.16]

DOI

10.37482/2687-1491-Z032

Authors

Anton V. Samoylov*/** ORCID: 0000-0001-5022-0448
Vladimir G. Solov’ev* ORCID: 0000-0003-4870-2282
Tat’yana Yu. Astakhova*/** ORCID: 0000-0002-8777-0686
*Khanty-Mansiysk State Medical Academy (Khanty-Mansiysk, Khanty-Mansi Autonomous Okrug – Yugra, Russian Federation)
**Khanty-Mansiysk District Clinical Hospital (Khanty-Mansiysk, Khanty-Mansi Autonomous Okrug – Yugra, Russian Federation)
Corresponding author: Anton Samoylov, address: ul. Mira 40, Khanty-Mansiysk, 628007, Khanty-Mansiyskiy avtonomnyy okrug – Yugra, Russian Federation; e-mail: acid2001@rambler.ru

Abstract

This study aimed to assess the state of haemostasis at supplementation of the diet with vitamins and the possibility of correcting changes in coagulation under combined thermal effects on the body of laboratory animals (white rats). The animals were kept on a nutritious diet with sufficient intake of proteins, fats, carbohydrates and trace elements. As part of daily servings in addition to the diet, the experimental group received vitamins A, E, B₆, B₉, B₁₂ and P in therapeutic doses adequate to those recommended for humans; the control group did not receive vitamins. After 14 days, part of the animals from both groups were subjected to combined thermal stress. At all painful manipulations the animals were anesthetized with ethoxyethane. Blood samples were taken in a syringe from the jugular vein exposed by an ovalshaped incision immediately after stress exposure. For coagulation testing, blood was stabilized with a 3.8 % sodium citrate solution in a ratio of 1 to 9. Subsequent blood treatment met the requirements accepted for coagulation testing. The following haemostatic parameters were studied: platelet count, activated partial thromboplastin time, prothrombin time, thrombin time, antithrombin III activity, as well as content of fibrinogen and soluble fibrin monomer complexes. The obtained results showed that the combined thermal effect causes a significant acceleration of continuous blood coagulation (consumption thrombocytopenia, hypofibrinogenaemia and mismatch of clotting test parameters) in rats. Additional vitaminization restrains the severity of these changes, which is manifested in the absence of platelet consumption, a decrease in the consumption of plasma coagulation factors and the preservation of a high antithrombin potential.

Keywords

haemostasis, platelets, thrombinogenesis, vitamins, hyperthermia, hypothermia, thermal stress, white rats

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