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Changes in the IR Spectra of Energy Metabolism Metabolites in the Blood of Rats with Hepatocellular Carcinoma Under the Influence of Hyperthermia and Apitoxin.C. 80-88

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Section: Biological sciences

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UDC

591.21:612.111.15

DOI

10.37482/2687-1491-Z170

Authors

Ol’ga V. Krasnikova* ORCID: https://orcid.org/0000-0002-4425-1819
Mikhail A. Shabalin** ORCID: https://orcid.org/0000-0003-2070-4948
Ol’ga V. Kondrashina* ORCID: https://orcid.org/0000-0002-9769-5173
Marina S. Piskunova* ORCID: https://orcid.org/0000-0002-4189-6259
Anastasiya R. Kondrat’eva* ORCID: https://orcid.org/0000-0001-8450-4537
Natal’ya V. Kol’tyukova* ORCID: https://orcid.org/0000-0003-4341-4476
Ol’ga M. Moskovtseva* ORCID: https://orcid.org/0000-0001-9806-5942

*Privolzhsky Research Medical University
(Nizhny Novgorod, Russian Federation)
**National Research Lobachevsky State University of Nizhny Novgorod
(Nizhny Novgorod, Russian Federation)

Corresponding author: Ol’ga Krasnikova, address: pl. Minina i Pozharskogo 10/1, Nizhny Novgorod, 603005, Russian Federation; e-mail: lala-g@yandex.ru

Abstract

There is a need to develop and improve conservative treatment methods for malignant liver tumours, since only in a small percentage of cases (10–30 %) radical treatments can be applied. Therefore, of relevance are studies into the effect of hyperthermia on the absorption of antitumour drugs and the effect of bee venom as a physiological therapeutic agent on the energy metabolism of tumour cells. Infrared spectroscopy could potentially be used to study changes in energy metabolism parameters in the blood plasma. The purpose of this article is to assess changes in the concentrations of energy metabolism metabolites in the blood of tumour-bearing animals during hyperthermia under the action of apitoxin using infrared spectroscopy. Materials and methods. The research involved 50 white non-linear female rats, divided into an intact, control and three experimental groups. The control and experimental groups were inoculated with hepatocellular carcinoma. The experimental groups were injected intraperitoneally with honey bee venom followed by hyperthermia sessions at different temperatures (42.5; 43.5 and 44.5 °С). Blood plasma was studied using a spectrophotometer in the wavenumber range of 1170–1025 cm–1. Results. We found a statistically significant increase in adenosine triphosphate concentrations (by 54 %) and a decrease in adenosine monophosphate (by 54 %), adenosine diphosphate (by 18 %) and glucose (by 87 %) levels compared to the control at 42.5 °C hyperthermia under the action of bee venom by the 7th day; however, on the 28th day the opposite effect was observed: an increase in adenosine monophosphate, adenosine diphosphate and glucose concentrations and a decrease in adenosine triphosphate levels. No positive dynamic was detected at other hyperthermia regimens. It is concluded that infrared spectroscopy can be used to assess energy changes in animals with hepatocellular carcinoma at different hyperthermia regimens under the therapeutic effect of bee venom. The optimal conditions for bee venom effects on the body are achieved at 42.5 °C.

Keywords

malignant liver tumours, hepatocellular carcinoma, bee venom, hyperthermia therapy, energy metabolism, infrared spectroscopy of blood serum

References

  1. Anwanwan D., Singh S.K., Singh S., Saikam V., Singh R. Challenges in Liver Cancer and Possible Treatment Approaches. Biochim. Biophys. Acta Rev. Cancer, 2020, vol. 1873, no. 1. Art. no. 188314. https://doi.org/10.1016/j.bbcan.2019.188314
  2. Alqahtani A., Khan Z., Alloghbi A., Said Ahmed T.S., Ashraf M., Hammouda D.M. Hepatocellular Carcinoma: Molecular Mechanisms and Targeted Therapies. Medicina (Kaunas), 2019, vol. 55, no. 9. Art. no. 526. https://doi.org/10.3390/medicina55090526
  3. Ademaj A., Veltsista D.P., Ghadjar P., Marder D., Oberacker E., Ott O.J., Wust P., Puric E., Hälg R.A., Rogers S., Bodis S., Fietkau R., Crezee H., Riesterer O. Clinical Evidence for Thermometric Parameters to Guide Hyperthermia Treatment. Cancers (Basel), 2022, vol. 14, no. 3. Art. no. 625. https://doi.org/10.3390/cancers14030625
  4. Crezee J., Franken N.A.P., Oei A.L. Hyperthermia-Based Anti-Cancer Treatments. Cancers (Basel), 2021, vol. 13, no. 6. Art. no. 1240. https://doi.org/10.3390/cancers13061240
  5. Dydykina V.N., Eremina Yu.D., Koryagin A.S., Smirnov V.P., Smirnova L.A. Vliyanie nanostrukturirovannykh sistem “Khitozan-nanochastitsy zolota”, “Khitozan-pchelinyy yad-nanochastitsy zolota” na strukturu i massu opukholi, perekisnoe okislenie lipidov i funktsional’noe sostoyanie krys s opukhol’yu RS-1 [Influence of Nanostructures Systems of “Chitosan-Gold Nanoparticles”, “Chitosan-Apitoxin-Gold Nanoparticles” on the Structure and Mass of the Tumor, Peroxidation of Lipids and Functional State of Rats Having RS-1 Tumor]. Meditsinskiy al’manakh, 2016, no. 2, pp. 133–137.
  6. Khalil A., Elesawy B.H., Ali T.M., Ahmed O.M. Bee Venom: From Venom to Drug. Molecules, 2021, vol. 26, no. 16. Art. no. 4941. https://doi.org/10.3390/molecules26164941
  7. Wehbe R., Frangieh J., Rima M., El Obeid D., Sabatier J.-M., Fajloun Z. Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests. Molecules, 2019, vol. 24, no. 16. Art. no. 2997. https://doi.org/10.3390/molecules24162997
  8. Sukhanova L.V., Kanarskiy A.V. Biologicheskaya tsennost’ pchelinovogo yada [Biological Value of Bee Venom]. Vestnik tekhnologicheskogo universiteta, 2016, vol. 19, no. 8, pp. 145–150.
  9. Korotkevich I.G., Borodin O.I. Strukturno-funktsional’nye svoystva i biologicheskaya aktivnost’ melitina iz yada pchel [Structure-Function Properties and Biological Activity of Melittine from Honey Bee Venom]. Trudy Belorusskogo gosudarstvennogo universiteta. Ser.: Fiziologicheskie, biokhimicheskie i molekulyarnye osnovy funktsionirovaniya biosistem, 2016, vol. 11, pt. 1, pp. 101–109.
  10. Butenko L.I., Kuleshova S.A., Podgornaya J.V., Myikots L.P., Dmitriev A.B. Рhysico-Chemical Studies of Apitoxin and Products on Its Basis. Pharm. Pharmacol., 2018, vol. 6, no. 4, pp. 351–366 (in Russ.). https://doi.org/10.19163/2307-9266-2018-6-4-351-366
  11. Kochan K., Bedolla D.E., Perez-Guaita D., Adegoke J.A., Chakkumpulakkal Puthan Veettil T., Martin M., Roy S., Pebotuwa S., Heraud P., Wood B.R. Infrared Spectroscopy of Blood. Appl. Spectrosc., 2021, vol. 75, no. 6, pp. 611–646. https://doi.org/10.1177/0003702820985856
  12. Gordetsov A.S. Infrakrasnaya spektroskopiya biologicheskikh zhidkostey i tkaney [Infrared Spectroscopy of Biological Fluids and Tissues]. Sovremennye tekhnologii v meditsine, 2010, no. 1, pp. 84–98.
  13. Krasnikova O.V. Fiziologicheskiy analiz infrakrasnykh spektrov plazmy krovi zhivotnykh v norme i pri eksperimental’nom onkogeneze [Physiological Analysis of Infrared Spectra of Animal Blood Plasma in Health and in Experimental Oncogenesis: Diss.]. Nizhny Novgorod, 2012. 133 p.
  14. Ancey P.-B., Contat C., Meylan E. Glucose Transporters in Cancer – from Tumor Cells to the Tumor Microenvironment. FEBS J., 2018, vol. 285, no. 16, pp. 2926–2943. https://doi.org/10.1111/febs.14577
  15. Krasnikova O.V., Gordetsov A.S., Krylov V.N. Izmenenie parametrov IK-spektrov plazmy krovi zhivotnykhopukholenositeley na fone vvedeniya biologicheski aktivnykh dobavok [The Change in the Parameters of Blood Plasma Infrared Spectra of Animals-Carriers of Tumours Against the Background of Dietary Supplement Administration]. Sovremennye tekhnologii v meditsine, 2011, no. 4, pp. 18–21.
  16. Krasnikova O.V., Gordetsov A.S., Kontorshchikova K.N., Krylov V.N., Sazanov A.I. Izmenenie parametrov IK-spektrov plazmy krovi zhivotnykh-opukholenositeley na fone kompleksnogo vvedeniya doksorubitsina i ozona [The Change in Parameters of Blood Plasma IR Spectra of Tumour-Bearing Animals on the Background of Doxorubicin and Ozone Complex Administration]. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo, 2011, no. 5-1, pp. 105–109.



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