UDC

[591.111:636.5:636.082.474.5](045)

DOI

https://doi.org/10.37482/2687-1491-Z283

Authors

Ulyana A. Krut* ORCID: https://orcid.org/0009-0009-6341-9750
Andrey A. Prisnyi*/** ORCID: https://orcid.org/0000-0001-5229-8337
Galina M. Shaidorova* ORCID: https://orcid.org/0000-0002-9467-027X
Sofya M. Ivanova* ORCID: https://orcid.org/0009-0007-6755-6686
Marina S. Potapova* ORCID: https://orcid.org/0009-0000-4642-0412
*Belgorod State National Research University (Belgorod, Russia)
**Belgorod Branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV” (Belgorod, Russia)
Corresponding author: Ulyana Krut, address: ul. Pobedy 85, Belgorod, 308015, Russia; e-mail: krut@bsuedu.ru

Abstract

The widespread use of antimicrobial drugs, such as enrofloxacin, in poultry farming is associated with risks of residue accumulation in the products and the development of antimicrobial resistance, posing a serious threat to consumer health. Probiotics, particularly those based on Bacillus subtilis, are considered a safe alternative capable of not only maintaining bird health but also improving the nutritional characteristics of the products. The purpose of this article was to conduct a comparative assessment of the effects of enrofloxacin, probiotic B. subtilis, and their combination on the haematological and biochemical blood parameters in egg-laying hybrid chickens as key indicators of the metabolic and immune status. Materials and methods. The research was conducted on Hisex Brown cockerels from one day to three weeks old (n = 100). Four groups of chickens were formed: a control group, a group that received the probiotic B. subtilis, a group that was given enrofloxacin, and a group that received a combination of the two. Blood sampling for biochemical and haematological analysis was performed on days 1, 5, 10 and 20 of the experiment. Results. The group that received enrofloxacin demonstrated a significant decrease in total protein and bilirubin levels on day 5, indicating liver dysfunction. In the groups that were given the probiotic, an increase in total protein by day 20 and a positive dynamic in lipid metabolism were observed. On day 10, these groups showed a significant decrease in pseudo-eosinophil concentration and an increase in lymphocyte levels, indicating stimulation of specific immunity. The combined use of the probiotic with the antibiotic mitigated some of the negative effects of the latter. The use of B. subtilis helped stabilize the biochemical status, optimize lipid metabolism and activate the specific immune response in chickens, in contrast to the negative impact of enrofloxacin. The obtained data confirm the potential for using probiotics to optimize poultry farming in order to enhance product safety and quality.

For citation: Krut U.A., Prisnyi A.A., Shaidorova G.M., Ivanova S.M., Potapova M.S. Haematological and Biochemical Blood Parameters in Egg-Laying Hybrid Chickens Under the Influence of a Probiotic Bacillus subtilis and Enrofloxacin. Journal of Medical and Biological Research, 2026, vol. 14, no. 2, pp. 36–46. DOI: 10.37482/2687-1491-Z283

Keywords

antimicrobial drugs, enrofloxacin, Bacillus subtilis, egg-laying hybrid chickens, blood biochemical parameters, haematological parameters, lipid metabolism, immune status

References

1. Bolotnikov I.A., Solov’ev Yu.V. Gematologiya ptits [Avian Haematology]. Leningrad, 1980. 116 p.

2. Ilyina G.V., Ilyin D.Yu., Grishina A.A., Dashkina A.R. Functional Complex of Microorganisms on a Mineral Carrier for Cascade Enzymatic Destruction of Poultry Waste. Agrar. Bull. Urals, 2024, vol. 24, no. 5, pp. 670–681 (in Russ.). https://doi.org/10.32417/1997-4868-2024-24-05-670-681

3. Akulov S.A., Chistyakova I.B., Fedotov A.A. Metody izmereniya urovnya gematokrita krovi [Methods for Measuring Blood Hematocrit]. Privolzhskiy nauchnyy vestnik, 2014, no. 11-1, pp. 29–32.

4. Lebedev S.V., Kazakova T.V., Marshinskaya O.V. Immunnyy status tsyplyat-broylerov v razlichnye fiziologicheskie periody razvitiya [Immune Status of Broiler Chickens at Different Stages of Physiological Development]. Vestnik rossiyskoy sel’skokhozyaystvennoy nauki, 2024, no. 3, pp. 92–96. https://doi.org/10.31857/S2500208224030205

5. Moiseeva A.A., Prisnyy A.A. Dinamika indeksa sootnosheniya psevdoeozinofilov i limfotsitov v krovi tsyplyat [Dynamics of the Pseudo-Eosinophil/Lymphocyte Ratio in the Blood of Chickens]. Trudy Vserossiyskogo NII eksperimental’noy veterinarii im. Ya.R. Kovalenko, 2018, vol. 80, no. 1, pp. 317–323.

6. Letkin A.I. Leykotsitarnye indeksy krovi kur-nesushek pri nespetsificheskom stressornom sindrome [Leukocytal Indices of Laying Hen Blood in Nonspecific Stress Syndrome]. Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta, 2020, no. 2, pp. 102–108.

7. Ostrovskiy V.K., Svitich Yu.M., Veber V.R. Leykotsitarnyy indeks intoksikatsii pri ostrykh gnoynykh i vospalitel’nykh zabolevaniyakh legkikh [Leukocyte Intoxication Index in Acute Purulent and Inflammatory Lung Diseases]. Vestnik khirurgii im. I.I. Grekova, 1983, vol. 131, no. 11, pp. 21–24.

8. Shcherbinina M.A., Kletikova L.V., Yakimenko N.N., Kokurina N.V. Diagnosticheskoe znachenie leykotsitarnykh indeksov v otsenke gomeostaza u tsyplyat posle vyvoda [Diagnostic Value of Leukocytal Indices in Assessing Homeostasis in Chickens After Hatching]. Sovremennye zadachi i perspektivnye napravleniya innovatsionnogo razvitiya agrarnoy nauki [Current Tasks and Promising Areas of Innovative Development of Agricultural Science]. Kurgan, 2024, pp. 106–110.

9. Moiseeva A.A., Skvortsov V.N., Prisnyy A.A. Pokazateli krasnoy krovi tsyplyat pri primenenii tsiprofloksatsina [Indicators of Chickens Red Blood Under the Effect of Ciprofloxacin]. Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy meditsiny im. N.E. Baumana, 2019, vol. 238, no. 2, pp. 124–128. https://doi.org/10.31588/2413-4201-1883-238-2-124-128

10. Dmitrieva O.S., Chelnokov A.A. Astsit u broylerov: lechebno-profilakticheskie meropriyatiya [Broiler Ascites: Therapeutics and Preventive Measures]. Izvestiya Velikolukskoy gosudarstvennoy sel’skokhozyaystvennoy akademii, 2023, no. 3, pp. 26–35.

11. Okolelova T.M., Engashev S.V., Egorov I.A., Egorova T.A. Role of Biochemical Blood Parameters in the Assessment of Physiological Status in Poultry. Ptitsevodstvo, 2023, no. 2, pp. 44–51 (in Russ.) https://doi.org/10.33845/0033-3239-2023-72-2-44-51

12. Śliżewska K., Markowiak P., Żbikowski A., Szeleszczuk P. Effects of Synbiotics on the Gut Microbiota, Blood and Rearing Parameters of Chickens. FEMS Microbiol. Lett., 2019, vol. 366, no. 11. Art. no. fnz116. https://doi.org/10.1093/femsle/fnz116

13. Reuben R.C., Sarkar S.L., Ibnat H., Roy P.C., Jahid I.K. Novel Mono- and Multi-Strain Probiotics Supplementation Modulates Growth, Intestinal Microflora Composition, and Haemato-Biochemical Parameters in Broiler Chickens. Vet. Med. Sci., 2022, vol. 8, no. 2, pp. 668–680. https://doi.org/10.1002/vms3.709

14. An K.V., Kil’p A.S., Mironova T.E., Afonyushkin V.N. Izuchenie immunologicheskikh reaktsiy u Mus musculus pri peroral’nom vvedenii Bacillus halotolerans i Bacillus subtilis [Study of Immune Responses in Mus musculus After Oral Administration of Bacillus halotolerans and Bacillus subtilis]. Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta, 2025, no. 2, pp. 56–62. https://doi.org/10.53083/1996-4277-2025-244-2-56-62

15. Duskaev G., Rakhmatullin S., Kvan O. Effects of Bacillus cereus and Coumarin on Growth Performance, Blood Biochemical Parameters, and Meat Quality in Broilers. Vet. World, 2020, vol. 13, no. 11, pp. 2484–2492. https://doi.org/10.14202/vetworld.2020.2484-2492